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HIV-1 RNA VIRAL LOAD BY REAL TIME-PCR [RT-PCR]

Technical Bulletins:

tb8671205b_20081205.pdf
tb8671205_20081205.pdf
tb8671205_20030116.doc
tb8671205_20021113.doc
tb8671205_20010725.doc

Test Information

Alternative Names:

•HIV-1 VIRAL LOAD
•HIV-1 RNA BY RT-PCR
•HIV VIRAL LOAD
•HIV VIRAL LOAD BY RT-PCR

KRMS Procedure Code: | 8671205
Health Connect Order Code: | HIV 1 RNA, QUANTITATIVE PCR [87536C]
CPT Code: | 87536
Methodology: | Real Time PCR [RT-PCR]
Performed At: | Regional Reference Laboratories, Sherman Way
Department: | Immunology
Testing Schedule: | Monday - Friday
Turn Around Time: | 2 days

Comments:

Note:
•No add-on requests from HIV-1 RNA Viral Laod by RT-PCR for additional HIV testing can be accepted due to different specimen requirements
•Please refer to Test Menu for specific Monogram HIV tests [i.e. GART, Phenotype, Trofile, etc.], which require two centrifuged and frozen PPT specimens

Specimen Collection Information
Specimen Container: | Two 5 mL Lavender EDTA [LAV5]
Collection Label Code: | VLOAD
Specimen Requirements: | Plasma from two LAV5
Minimum Test Volume: | 2.0 mL
Collection Instructions: | Draw two 5 mL Lavender Top EDTA [LAV5]. DO NOT FREEZE, prior to transport to the Regional Reference Laboratories.
Pre-Analytical Handling and Transport

Processing Instructions:

•Centrifuge EDTA tubes
•Aliquot the plasma from both EDTA tubes into a sterile polypropylene tube within 6 hours of collection
•Immediately refrigerate plasma aliquot at 2-8°C
•Send refrigerated plasma to the RRL Specimen Processing Department on the same day of collection

Transport Temperature: | Refrigerated
Specimen Stability Ambient: | 1 day
Specimen Stability Refrigerated: | 5 days
Specimen Stability Frozen: | 1 month [after testing is performed]

Rejection Criteria:

•Samples received not aliquoted
•Samples frozen as whole blood [uncentrifuged]
•Samples with incomplete seperation of red cells and plasma
•Unlabeled samples
•Improperly collected or transported specimens
•More than 5 freeze/thaw cycles of sample is unacceptable

Result Interpretation and Clinical Significance

Result Interpretation:

•The lowest reportable value is 48 copies/mL, limit of detection
•Samples with <48 copies/mL are below the limit of quantitation of the assay
•Samples with values of>10,000,000 copies/mL are above the upper limit of detection and will be reported as>10,000,000 copies/mL

Reference Range:

Patient values below the limit of detection are reported as <48 copies/mL.

Clinical Significance: | Aid in the management of individuals infected with HIV-1 on anti-retroviral therapy.

RAROSSER / 2010-11-08 15:31:30.0

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